TAGMe DNA Methylation Kits (qPCR) oo loogu talagalay Kansarka Makaanka afkiisa
MUUQAALKA WAX soo saarka
Saxnaanta
La ansixiyay in ka badan 36000 muunado caafimaad oo laba-indho-indho la'aan ah oo xarumo badan leh, alaabtu waxay leedahay gaar ahaan 94.3% iyo xasaasiyad 96.0%.
Ku habboon
Farsamada ogaanshaha Me-qPCR ee asalka ah waxaa lagu dhammeyn karaa hal tallaabo gudahood 3 saacadood iyada oo aan la beddelin bisulfit.
Horey
Baaritaanka kansarka ilmagaleenka ilmo-galeenka waxaa loo gudbi karaa marxaladda nabarro heer sare ah (nabarrada hore ee kansarka).
Automation
Oo ay weheliso sooftiweerka falanqaynta natiijada la habeeyey, tarjumaada natiijooyinka waa mid toos ah oo si toos ah loo akhriyi karo.
ISTICMAALKA KULA JIRA
Qalabkan waxa loo isticmaalaa in vitro lagu ogaado tayada hypermethylation ee hiddasidaha PCDHGB7 ee muunadaha afka ilmagaleenka.Natiijadu waxay muujinaysaa khatarta sii kordheysa ee fasalka 2 ama heerka sare/intraepithelial neoplasia ilmo-galeenka ilmo-galeenka oo sii horumarsan (CIN2+, oo ay ku jiraan CIN2, CIN3, adenocarcinoma in situ, iyo kansarka ilmo-galeenka), kaas oo u baahan baaritaan dheeri ah iyo/ama baaritaanka histopathological.Taas beddelkeeda, natiijooyinka imtixaannada taban waxay muujinayaan in khatarta CIN2+ ay yar tahay, laakiin khatarta si buuxda looma saari karo.Cilad-baadhista kama dambaysta ah waa in lagu saleeyaa colposcopy iyo/ama natiijooyinka histopathological.PCDHGB7 waa xubin ka tirsan qoyska protocadherin γ kooxda hidde-sideyaasha.Protocadherin ayaa la ogaaday in ay nidaamiso hababka noolaha sida kororka unugyada, wareegga unugyada, apoptosis, duulaanka, socdaalka iyo autophagy ee unugyada burooyinka iyada oo loo marayo dariiqooyin calaamado kala duwan, iyo aamusnaanta hidda-wadaha oo ay sababtay hypermethylation ee gobolka dhiirrigeliyaha ayaa si dhow ula xiriira dhacdada iyo horumarka. Kansarro badan.Waxaa la sheegay in hypermethylation of PCDHGB7 ay la xiriirto noocyo kala duwan oo burooyin ah, sida non-Hodgkin lymphoma, kansarka naasaha, kansarka ilmo-galeenka, kansarka endometrial iyo kansarka kaadiheysta.
Mabda'a Ogaanshaha
Qalabkani waxa uu ka kooban yahay reagent soo saarista aashitada nucleic iyo PCR reagen.Aashitada Nucleic-ka waxaa lagu soo saaraa habka birlab-ku-salaysan.Xirmadani waxay ku salaysan tahay mabda'a habka PCR-ga ee fluorescence-ka, iyadoo la isticmaalayo methylation-gaar ah waqtiga-dhabta ah ee PCR falcelinta si loo falanqeeyo template DNA, isla mar ahaantaana la ogaado goobaha CpG ee hidda-wadaha PCDHGB7 iyo calaamadaynta tayada xakamaynta tayada ee jajabyada hidda-wadaha G1 iyo G2.Heerka methylation ee PCDHGB7 ee muunada, ama qiimaha Me, waxaa loo xisaabiyaa si waafaqsan PCDHGB7 hiddasidaha methylated DNA-ga qiimaha Ct iyo qiimaha Ct ee tixraaca.Heerka PCDHGB7 hypermethylation togan ama xaalad xun waxaa lagu go'aamiyaa iyadoo loo eegayo qiimaha Me.
Xaaladaha codsiga
Baaritaanka hore
Dadka caafimaadka qaba
Qiimaynta Khatarta Kansarka
Dadka khatarta sare leh (waxay u fiican yihiin fayraska papillomavirus ee khatarta sare leh (hrHPV) ama u fiican cytology exfoliation ilmo galeenka)
Kormeerka soo noqnoqda
Dadka qalliinka kadib (oo leh taariikh nabarro heer sare ah oo makaanka afkiisa ah ama kansarka ilmo galeenka)
Muhiimada caafimaad
Baaritaanka hore ee dadka caafimaadka qaba:Kansarka afka ilmo-galeenka iyo nabarrada kansarka ka horreeya ayaa si sax ah loo baari karaa
Qiimaynta khatarta ah ee dadka khatarta sare leh:Kala soocida khatarta ah waxaa lagu samayn karaa dadka qaba HPV si loo hago ogaanshaha kala soocida dambe
Kormeerka soo noqnoqda ee dadka qalliinka kadib:La socodka soo noqoshada dadka ee qalliinka ka dib ayaa la samayn karaa si looga hortago dib u dhac ku yimaada daaweynta oo ay keento soo noqoshada
Tusaalaha ururinta
Habka muunada: Dhig muunada afka ilmo-galeenka ee la tuuri karo os-ka ilmo-galeenka, si tartiib ah u xoq buraashka ilmo-galeenka oo u wareeji 4-5 jeer saacadda, si tartiib ah uga saar burushka ilmo-galeenka, geli xalka ilaalinta unugyada, oo ku calaamadee baaritaanka soo socda.
Ilaalinta shaybaarada:Muunada waxaa lagu kaydin karaa heerkulka qolka ilaa 14 maalmood, 2-8 ℃ ilaa 2 bilood, iyo at -20± 5℃ ilaa 24 bilood.
Habka ogaanshaha: 3 saacadood (la'aanteed habka gacanta)
Xirmooyinka ogaanshaha Methylation DNA (qPCR) ee Kansarka Urothelial
| Codsiga bukaan socodka | Baaritaanka caawinta kiliinikada ee kansarka ilmo galeenka |
| Hidde-helidda | PCDHGB7 |
| Tusaalaha nooca | Muunadaha ilmagaleenka dheddigga |
| Habka tijaabada | Tiknoolajiyada PCR ee cabirka fluorescence |
| Qaabka lagu dabaqi karo | ABI7500 |
| Tilmaamaha xirxirida | 48 tijaabo/xirmo |
| Xaaladaha Kaydinta | Qalabka A waa in lagu kaydiyaa 2-30 ℃ Qalabka B waa in lagu kaydiyaa -20±5℃ Shaqeynaya ilaa 12 bilood |
