TAGMe DNA Methylation Kits (qPCR) oo loogu talagalay Kansarka Ilma-galeenka / Kansarka Endometrial
MUUQAALKA WAX soo saarka
Aan duullaan ahayn
Lagu dabaqi karo burushka afka ilmagaleenka iyo shaybaarka Pap smear.
Ku habboon
Farsamada ogaanshaha Me-qPCR ee asalka ah waxaa lagu dhammeyn karaa hal tallaabo gudahood 3 saacadood iyada oo aan la beddelin bisulfit.
Horey
Lagu ogaan karo marxaladda kansarka hore.
Automation
Oo ay weheliso sooftiweerka falanqaynta natiijada la habeeyey, tarjumaada natiijooyinka waa mid toos ah oo si toos ah loo akhriyi karo.
Xaaladaha codsiga
Baaritaanka hore
Dadka caafimaadka qaba
Qiimaynta Khatarta Kansarka
Dadka khatarta sare leh (oo u wanaagsan papillomavirus-ka khatarta sare leh (hrHPV) ama u fiican cytology exfoliation ilmo-galeenka / togan ee khatarta sare ee papillomavirus (hrHPV) ama u fiican cytology exfoliation ilmo-galeenka)
Kormeerka soo noqnoqda
Dadka saadaaliya
ISTICMAALKA KULA JIRA
Qalabkan waxa loo isticmaalaa in vitro lagu ogaado tayada hypermethylation ee hiddasidaha PCDHGB7 ee muunadaha afka ilmagaleenka.Kansarka ilmo-galeenka, natiijada togan waxay muujinaysaa khatarta sii kordheysa ee fasalka 2 ama heerka sare/intraepithelial neoplasia ilmo-galeenka ilmo-galeenka oo aad u horumarsan (CIN2+, oo ay ku jiraan CIN2, CIN3, adenocarcinoma in situ, iyo kansarka ilmo-galeenka), kaas oo u baahan baaritaan dheeraad ah colposcopy iyo/ama baaritaanka histopathological. .Taas beddelkeeda, natiijooyinka imtixaannada taban waxay muujinayaan in khatarta CIN2+ ay yar tahay, laakiin khatarta si buuxda looma saari karo.Cilad-baadhista kama dambaysta ah waa in lagu saleeyaa colposcopy iyo/ama natiijooyinka histopathological.Intaa waxaa dheer, kansarka endometrial, natiijada togan waxay muujinaysaa khatarta sii kordheysa ee nabarrada precancerous endometrial iyo kansarka, taas oo u baahan baaritaan dheeraad ah histopathological ee endometrium.Taas beddelkeeda, natiijooyinka baaritaanka taban waxay muujinayaan in halista nabarrada kansarka ee endometrial-ka iyo kansarku ay yar yihiin, laakiin khatarta gebi ahaanba lagama saari karo.Cilad-baadhista kama dambaysta ah waa in lagu saleeyaa natiijooyinka baaritaanka histopathological ee endometrium.
PCDHGB7 waa xubin ka tirsan qoyska protocadherin γ kooxda hidde-sideyaasha.Protocadherin ayaa la ogaaday in ay nidaamiso hababka noolaha sida kororka unugyada, wareegga unugyada, apoptosis, duulaanka, socdaalka iyo autophagy ee unugyada burooyinka iyada oo loo marayo dariiqooyin calaamado kala duwan, iyo aamusnaanta hidda-wadaha oo ay sababtay hypermethylation ee gobolka dhiirrigeliyaha ayaa si dhow ula xiriira dhacdada iyo horumarka. Kansarro badan.Waxaa la sheegay in hypermethylation of PCDHGB7 ay la xiriirto noocyo kala duwan oo burooyin ah, sida non-Hodgkin lymphoma, kansarka naasaha, kansarka ilmo-galeenka, kansarka endometrial iyo kansarka kaadiheysta.
Mabda'a Ogaanshaha
Qalabkani waxa uu ka kooban yahay reagent soo saarista aashitada nucleic iyo PCR reagen.Aashitada Nucleic-ka waxaa lagu soo saaraa habka birlab-ku-salaysan.Xirmadani waxay ku salaysan tahay mabda'a habka PCR-ga ee fluorescence-ka, iyadoo la isticmaalayo methylation-gaar ah waqtiga-dhabta ah ee PCR falcelinta si loo falanqeeyo template DNA, isla mar ahaantaana la ogaado goobaha CpG ee hidda-wadaha PCDHGB7 iyo calaamadaynta tayada xakamaynta tayada ee jajabyada hidda-wadaha G1 iyo G2.Heerka methylation ee PCDHGB7 ee muunada, ama qiimaha Me, waxaa loo xisaabiyaa si waafaqsan PCDHGB7 hiddasidaha methylated DNA-ga qiimaha Ct iyo qiimaha Ct ee tixraaca.Heerka PCDHGB7 hypermethylation togan ama xaalad xun waxaa lagu go'aamiyaa iyadoo loo eegayo qiimaha Me.
