Xirmooyinka ogaanshaha Methylation DNA TAGMe (qPCR) ee Kansarka kaadi mareenka
MUUQAALKA WAX soo saarka
Saxnaanta
La ansixiyay in ka badan 3500 oo muunado caafimaad ah oo lagu sameeyay daraasado xarumo badan oo laba-indho-indho ah, alaabtu waxay leedahay gaar ahaan 92.7% iyo xasaasiyad 82.1%.
Ku habboon
Farsamada ogaanshaha Me-qPCR ee asalka ah waxaa lagu dhammeyn karaa hal tallaabo gudahood 3 saacadood iyada oo aan la beddelin bisulfit.
Aan duullaan ahayn
Kaliya 30 ml oo muunad kaadi ah ayaa loo baahan yahay si loo ogaado 3 nooc oo kansar ah, oo ay ku jiraan kansarka miskaha kelyaha, kansarka kaadi mareenka, kansarka kaadiheysta isku mar.
Xaaladaha codsiga
Ogaanshaha Kaaliyaha
Dadka la ildaran xanuunka hematuria/ looga shakisan yahay inay qabaan kaadi mareenka (kansarka kaadi mareenka/kansarka miskaha kelyaha)
Qiimaynta Khatarta Kansarka
Qalliin/kemotherabi- u baahan dadka qaba kansarka kaadi mareenka;
Kormeerka soo noqnoqda
Dadka qalliinka kadib qaba kansarka kaadi mareenka
ISTICMAALKA KULA JIRA
Xirmadan waxa loo istcimaalaa ogaanshaha tayada ee vitro ee hypermethylation of the Urothelial Carcinoma(UC) hiddasidaha shaybaarada kaadi mareenka.Natiijadu waxay muujinaysaa khatarta sii kordheysa ee UC, taas oo u baahan cystoscope dheeraad ah iyo/ama baaritaanka histopathological.Taas beddelkeeda, natiijooyinka imtixaannada taban waxay muujinayaan in khatarta UC ay yar tahay, laakiin khatarta si buuxda looma saari karo.Baaritaanka ugu dambeeya waa inuu ku salaysan yahay cystoscope iyo/ama natiijooyinka histopathological.
Mabda'a Ogaanshaha
Qalabkani waxa uu ka kooban yahay reagent soo saarista aashitada nucleic iyo PCR reagen.Aashitada Nucleic-ka waxaa lagu soo saaraa habka birlab-ku-salaysan.Qalabkani wuxuu ku salaysan yahay mabda'a habka PCR-ga ee fluorescence-ka, iyadoo la adeegsanayo methylation-gaar ah-waqtiga dhabta ah ee PCR falcelinta si loo falanqeeyo DNA-da, isla mar ahaantaana lagu ogaado goobaha CpG ee hidda-wadaha UC iyo calaamadaynta tayada xakamaynta tayada ee jajabyada hidda-wadaha G1 iyo G2.Heerarka methylation ee hiddo-wadaha UC, oo loo yaqaan qiimaha Me, waxaa loo xisaabiyaa iyadoo loo eegayo hidda-wadaha UC methylated DNA amplification qiimaha Ct iyo qiimaha Ct ee tixraaca.Heerarka UC ee hypermethylation togan ama heerka taban waxaa lagu go'aamiyaa iyadoo loo eegayo qiimaha Me.
Xirmooyinka ogaanshaha Methylation DNA (qPCR) ee Kansarka Urothelial
| Codsiga bukaan socodka | Ogaanshaha kaaliyaha kiliinikada ee kansarka kaadi mareenka;Qiimaynta waxtarka daaweynta qaliinka/chemotheray;la socodka soo noqoshada qalliinka kadib |
| Hidde-helidda | UC |
| Tusaalaha nooca | Muunadda unugga kaadida ee dibadda loo saaro (sediment kaadi) |
| Habka tijaabada | Tiknoolajiyada PCR ee cabirka fluorescence |
| Moodooyinka lagu dabaqi karo | ABI7500 |
| Tilmaamaha xirxirida | 48 tijaabo/xirmo |
| Xaaladaha Kaydinta | Qalabka A waa in lagu kaydiyaa 2-30 ℃ Qalabka B waa in lagu kaydiyaa -20±5℃ Shaqeynaya ilaa 12 bilood |
