TAGMe DNA Methylation Kits (qPCR) oo loogu talagalay Kansarka Endometrial
MUUQAALKA WAX soo saarka
Saxnaanta
La ansixiyay in ka badan 800 oo muunado bukaan-socod ah oo lagu sameeyay daraasado xarumo badan oo laba-indho-indho ah, alaabtu waxay leedahay gaar ahaan 82.81% iyo dareen 80.65%.
Ku habboon
Farsamada ogaanshaha Me-qPCR ee asalka ah waxaa lagu dhammeyn karaa hal tallaabo gudahood 3 saacadood iyada oo aan la beddelin bisulfit.
Horey
Lagu ogaan karo marxaladda kansarka hore.
Automation
Lagu dabaqi karo burushka afka ilmagaleenka iyo shaybaarka Pap smear.
ISTICMAALKA KULA JIRA
Qalabkan waxa loo isticmaalaa in vitro lagu ogaado tayada hypermethylation ee DNA-da PCDHGB7 muunadaha ilmo-galeenka.Natiijo wanaagsan ayaa muujinaysa khatarta sii kordheysa ee nabarrada kansarka hore ee endometrial iyo kansarka, taas oo u baahan baaritaan dheeri ah oo histopathological endometrium.Taas beddelkeeda, natiijooyinka baaritaanka taban waxay muujinayaan in halista nabarrada kansarka ee endometrial-ka iyo kansarku ay yar yihiin, laakiin khatarta gebi ahaanba lagama saari karo.Cilad-baadhista kama dambaysta ah waa in lagu saleeyaa natiijooyinka baaritaanka histopathological ee endometrium.PCDHGB7 waa xubin ka tirsan qoyska protocadherin γ kooxda hidde-sideyaasha.Protocadherin ayaa la ogaaday in ay nidaamiso hababka noolaha sida kororka unugyada, wareegga unugyada, apoptosis, duulaanka, socdaalka iyo autophagy ee unugyada burooyinka iyada oo loo marayo dariiqooyin calaamado kala duwan, iyo aamusnaanta hidda-wadaha oo ay sababtay hypermethylation ee gobolka dhiirrigeliyaha ayaa si dhow ula xiriira dhacdada iyo horumarka. Kansarro badan.Waxaa la sheegay in hypermethylation of PCDHGB7 ay la xiriirto noocyo kala duwan oo burooyin ah, sida non-Hodgkin lymphoma, kansarka naasaha, kansarka ilmo-galeenka, kansarka endometrial iyo kansarka kaadiheysta.
Mabda'a Ogaanshaha
Qalabkani waxa uu ka kooban yahay reagent soo saarista aashitada nucleic iyo PCR reagen.Aashitada Nucleic-ka waxaa lagu soo saaraa habka birlab-ku-salaysan.Xirmadani waxay ku salaysan tahay mabda'a habka PCR-ga ee fluorescence-ka, iyadoo la isticmaalayo methylation-gaar ah waqtiga-dhabta ah ee PCR falcelinta si loo falanqeeyo template DNA, isla mar ahaantaana la ogaado goobaha CpG ee hidda-wadaha PCDHGB7 iyo calaamadaynta tayada xakamaynta tayada ee jajabyada hidda-wadaha G1 iyo G2.Heerka methylation ee PCDHGB7 ee muunada, ama qiimaha Me, waxaa loo xisaabiyaa si waafaqsan PCDHGB7 hiddasidaha methylated DNA-ga qiimaha Ct iyo qiimaha Ct ee tixraaca.Heerka PCDHGB7 hypermethylation togan ama xaalad xun waxaa lagu go'aamiyaa iyadoo loo eegayo qiimaha Me.
Xaaladaha codsiga
Baaritaanka hore
Dadka caafimaadka qaba
Qiimaynta Khatarta Kansarka
Kooxaha khatarta sare leh (dadka leh dhiig-bax aan caadi ahayn siilka ka dib menopause, dhumucda endometrial, iwm.)
Kormeerka soo noqnoqda
Dadka saadaaliya
Muhiimada caafimaad
Baaritaanka hore ee dadka caafimaadka qaba:Kansarka endometrial iyo nabarrada kansarka hore ayaa si sax ah loo baari karaa;
Qiimaynta khatarta ah ee dadka khatarta sare leh:Qiimaynta khatarta ah waxaa loo samayn karaa dadka qaba dhiigbaxa siilka ee aan caadiga ahayn iyo dhumucda endometrial ka dib menopause si looga caawiyo ogaanshaha caafimaad;
Korjoogteynta soo noqnoqoshada dadweynaha ee saadaasha:La socodka soo noqoshada dadka ee qalliinka kadib waa la samayn karaa si looga hortago dib u dhac ku yimaada daawaynta oo ay keento soo noqoshada.
Tusaalaha ururinta
Habka muunada: Dhig muunada afka ilmo-galeenka ee la tuuri karo os-ka ilmo-galeenka, si tartiib ah u xoq buraashka ilmo-galeenka oo u wareeji 4-5 jeer saacadda, si tartiib ah uga saar burushka ilmo-galeenka, geli xalka ilaalinta unugyada, oo ku calaamadee baaritaanka soo socda.
Ilaalinta shaybaarada:Muunada waxaa lagu kaydin karaa heerkulka qolka ilaa 14 maalmood, 2-8 ℃ ilaa 2 bilood, iyo at -20± 5℃ ilaa 24 bilood.
Habka ogaanshaha: 3 saacadood (la'aanteed habka gacanta)
TAGMe DNA Methylation Kits (qPCR) oo loogu talagalay Kansarka Endometrial
| Codsiga bukaan socodka | Baarista kaaliyaha kiliinikada ee kansarka endometrial |
| Hidde-helidda | PCDHGB7 |
| Tusaalaha nooca | Muunadaha ilmagaleenka dheddigga |
| Habka tijaabada | Tiknoolajiyada PCR ee cabirka fluorescence |
| Moodooyinka lagu dabaqi karo | ABI7500 |
| Tilmaamaha xirxirida | 48 tijaabo/xirmo |
| Xaaladaha Kaydinta | Qalabka A waa in lagu kaydiyaa 2-30 ℃ Qalabka B waa in lagu kaydiyaa -20±5℃ Shaqeynaya ilaa 12 bilood |
Nagu saabsan
Epiprobe waxay leedahay dhismo kaabayaal oo dhammaystiran: Xarunta wax soo saarka GMP waxay dabooshaa aag dhan 2200 mitir oo laba jibbaaran, waxayna ilaalisaa nidaamka maaraynta tayada ISO13485, kaas oo buuxiya shuruudaha wax soo saarka ee dhammaan noocyada alaabada reagent ee tijaabada hidda-socodka;Shaybaarka caafimaadku wuxuu daboolayaa dhul dhan 5400 mitir oo laba jibbaaran wuxuuna awood u leeyahay inuu fuliyo ganacsiga ogaanshaha methylation kansarka sida shaybaar caafimaad oo dhinac saddexaad ah oo shahaadeysan.Ka sokow, waxaan haynaa saddex badeeco oo la helay shahaadada CE, oo daboolaysa kansarka ilmo-galeenka, kansarka endometrial iyo ogaanshaha kansarka kaadi mareenka.
Tignoolajiyada ogaanshaha molecular ee kansarka Epiprobe waxaa loo isticmaali karaa baaritaanka kansarka hore, ogaanshaha kaaliyaha, qiimeynta qalliinka ka hor iyo qalliinka kadib, la socodka dib-u-soo-kabashada, kaas oo socodsiiya dhammaan habka ogaanshaha kansarka iyo daaweynta, siinta xalal wanaagsan dhakhaatiirta iyo bukaanka.
