Xirmada Soosaarka Ashitada Nukliyeerka (A02)
Mabda'a ogaanshaha
Ka dib marka la sii daayo DNA-da genomic iyada oo la kala qaybinayo unugyo leh bakhaar lysis ah, kuul birlabku wuxuu si xushmad leh ugu xidhi karaa DNA-da genomic ee muunadda.Tiro yar oo wasakh ah oo uu nuugo kuul birlabeedka ayaa laga saari karaa kaydiyaha.Gudaha TE, kuul birlabku wuxuu sii deyn karaa DNA-da boundgenome, isagoo helaya DNA-ga genome-ga tayada sare leh.Habkani waa mid fudud oo degdeg ah tayada DNA-da ee la soo saarayna waa mid sare, taas oo buuxin karta shuruudaha lagu ogaanayo methyl DNA.Dhanka kale, xirmada soo saarista ee ku salaysan kuul birlabeedka ayaa la jaan qaadi kara soo saarista aashitada nucleic-ka ah ee tooska ah, oo buuxinaysa hawlaha soo saarista aashitada nucleic-ka-sare ee soo saarista.
Qaybaha ugu muhiimsan ee reagent
Qaybaha waxaa lagu muujiyay shaxda 1:
Shaxda 1 Qaybaha Reagent iyo Soodejinta
| Magaca qaybta | Qaybaha ugu muhiimsan | Cabbirka (48) | Cabbirka (200) |
| 1. Dheefshiidka Buffer A | Tris, SDS | 15.8 ml/ dhalo | 66ml/ dhalo |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 ml/ dhalo | 66ml/ dhalo |
| 3. Bakhaarka dhaqda A | NaCl, Tris | 11 ml / dhalo | 44 ml / dhalo |
| 4. Meesha lagu dhaqo B | NaCl, Tris | 13 ml / dhalo | 26.5ml/ dhalo *2 |
| 5. TE | Tris, EDTA | 12 ml/dhalo | 44 ml / dhalo |
| 6. Protease K xal | Protease K | 1.1mL / gabal | 4.4ml / gabal |
| 7. Joojinta birta birta ah ee birta 2 | Kuul magnetic | 0.5mL / gabal | 2.2mL / gabal |
| 8. Tilmaamaha soo saarida reagents nucleic acid | / | 1 koobi | 1 koobi |
Qaybaha looga baahan yahay soo saarista aashitada nucleic, laakiin aan lagu darin xirmada:
1. Reagent: ethanol anhydrous, isopropanol, iyo PBS;
2. Alaabta: 50mL tuubada centrifuge iyo 1.5mL tuubada EP;
3. Qalabka: Biyaha qubeyska, pipettes, shelf magnetic, centrifuge, saxan 96-well (otomaatig ah), qalabka soo saarista nucleic acid (otomaatig ah).
Macluumaadka aasaasiga ah
Shuruudaha Tusaalaha:
1. Ogaanshaha waa in lagu dhammeeyaa kaydinta 7-maalmood ee heerkulka jawiga ka dib ururinta muunada unugga exfoliated ee ilmo-galeenka (aan-go'an).
2. Ogaanshaha waa in lagu dhammeeyaa kaydinta 30-maalmood ee heerkulka jawiga ka dib ururinta muunada unugga exfoliated ee ilmo-galeenka (go'an).
3. Ogaanshaha waa in lagu dhammeeyaa kaydinta 30-maalmood ee heerkulka deegaanka ka dib ururinta muunada kaadida;Ogaanshaha waa in la dhammeeyaa wakhtiga ka dib marka la ururiyo shaybaarada unugga la dhaqan geliyay.
Tilmaamaha baarkinka:200 pcs/sanduuq, 48 pcs/sanduuq.
Xaaladaha kaydinta:2-30℃
Muddada ansaxnimada:12 bilood
Qalabka lagu dabaqi karo:Tianlong NP968-C nucleic acid qalab soo saarista, Tiangen TGuide S96 qalabka saarista nucleic acid, GENE DIAN EB-1000 qalabka saarista nucleic acid.
Shahaadada diiwaanka qalabka caafimaadka Maya/waxyaabaha looga baahan yahay farsamo Maya.HJXB No. 20210100.
Taariikhda oggolaanshaha iyo dib-u-eegista tilmaamaha:Taariikhda ansixinta: Noofambar 18, 2021
Nagu saabsan
Sida shirkad tignoolajiyada sare ah oo lagu aasaasay 2018 khubarada epigenetic ee ugu sareysa, Epiprobe waxay diiradda saartaa ogaanshaha molecular ee methylation DNA-ga kansarka iyo warshadaha daweynta saxda ah.Iyada oo ku saleysan tignoolajiyada qoto dheer, waxaan hiigsaneynaa in aan horseedno waaga wax soo saarka cusub si ay u nuugaan kansarka birta!
Iyada oo ku saleysan cilmi-baarista muddada-dheer ee kooxda xudunta u ah Epiprobe, horumarinta iyo isbeddelka berrinka DNA-da methylation oo leh hal-abuurnimo-goynta, oo ay weheliso bartilmaameedyada methylation DNA ee gaarka ah ee kansarrada, waxaan isticmaalnaa algorithm kala duwanaansho gaar ah oo isku daraya xog weyn iyo tignoolajiyada sirdoonka macmal si madaxbanaan u horumariya tignoolajiyada dheecaanka-qaadista ee dareeraha ah ee gaarka ah.Iyadoo la falanqeynayo heerka methylation ee goobo gaar ah oo ka mid ah jajabyada DNA-da ee bilaashka ah ee muunada, cilladaha hababka baaritaanka dhaqameed iyo xaddidaadaha qalliinka iyo muunada daloolka ayaa la iska ilaaliyaa, taas oo aan kaliya lagu gaari karin ogaanshaha saxda ah ee kansarrada hore, laakiin sidoo kale waxay awood u siineysaa kormeerka waqtiga dhabta ah. dhacdooyinka kansarka iyo dhaqdhaqaaqa horumarka.
