Xirmada Soosaarka Ashitada Nukliyeerka (A01)
Mabda'a ogaanshaha
Ka dib marka la sii daayo DNA-da genomic iyada oo la kala qaybinayo unugyo leh bakhaar lysis ah, kuul birlabku wuxuu si xushmad leh ugu xidhi karaa DNA-da genomic ee muunadda.Tiro yar oo wasakh ah oo uu nuugo kuul birlabeedka ayaa laga saari karaa kaydiyaha.Gudaha TE, kuul birlabku wuxuu sii deyn karaa DNA-da boundgenome, isagoo helaya DNA-ga genome-ga tayada sare leh.Habkani waa mid fudud oo degdeg ah tayada DNA-da ee la soo saarayna waa mid sare, taas oo buuxin karta shuruudaha lagu ogaanayo methyl DNA.Dhanka kale, xirmada soo saarista ee ku salaysan kuul birlabeedka ayaa la jaan qaadi kara soo saarista aashitada nucleic-ka ah ee tooska ah, oo buuxinaysa hawlaha soo saarista aashitada nucleic-ka-sare ee soo saarista.
Qaybaha ugu muhiimsan ee reagent
Qaybaha waxaa lagu muujiyay shaxda 1:
Shaxda 1 Qaybaha Reagent iyo Soodejinta
| Magaca qaybta | Qaybaha ugu muhiimsan | Cabbirka (48) | Cabbirka (200) |
| 1. Xalka Lysis | Guanidine hydrochloride, Tris | 11 ml / dhalo | 44 ml / dhalo |
| 2. Xalalka nadiifinta A | NaCl, Tris | 11 ml / dhalo | 44 ml / dhalo |
| 3. Xalalka nadiifinta B | NaCl, Tris | 13 ml / dhalo | 26.5ml/ dhalo *2 |
| 4. Eluent | Tris, EDTA | 12 ml/dhalo | 44 ml / dhalo |
| 5. Protease K xal | Protease K | 1.1mL / gabal | 4.4ml / gabal |
| 6. Joojinta birta birta ah ee birta 1 | Kuul magnetic | 1.1mL / gabal | 4.4ml / gabal |
| 7. Tilmaamaha soo saarida reagents nucleic acid |
| 1 koobi | 1 koobi |
Qaybaha looga baahan yahay soo saarista aashitada nucleic, laakiin aan lagu darin xirmada:
1. Reagent: ethanol anhydrous, isopropanol iyo PBS;
2. Alaabta: 50ml tuubo centrifuge iyo 1.5ml tuubada EP;
3. Qalabka: Kiliga biyaha qubeyska, pipettor, shelf magnetka, centrifuge, saxan qoto dheer oo 96-dalool ah (otomaatig ah), qalabka soo saarista aashitada nucleic ee tooska ah (otomaatig ah).
Macluumaadka aasaasiga ah
Shuruudaha Tusaalaha
1. Wasakhda isdhaafka ah ee u dhaxaysa shaybaarada waa in laga fogaadaa ururinta iyo kaydinta muunada.
2. Ogaanshaha waa in lagu dhammeeyaa kaydinta 7-maalmood ee heerkulka jawiga ka dib ururinta muunada unugga exfoliated ee ilmo-galeenka (aan-go'an).Ogaanshaha waa in lagu dhammeeyaa kaydinta 30 maalmood ee heerkulka deegaanka ka dib ururinta muunada kaadida;Ogaanshaha waa in la dhammeeyaa wakhtiga ka dib marka la ururiyo shaybaarada unugga la dhaqan geliyay.
Tilmaamaha baarkinka:200 pcs/sanduuq, 48 pcs/sanduuq.
Xaaladaha kaydinta:2-30℃
Muddada ansaxnimada:12 bilood
Qalabka lagu dabaqi karo:Tianlong NP968-C nucleic acid qalab soo saarista, Tiangen TGuide S96 qalabka saarista nucleic acid, GENE DIAN EB-1000 qalabka saarista nucleic acid.
Shahaadada diiwaanka qalabka caafimaadka Maya/waxyaabaha looga baahan yahay farsamo Maya. HJXB No. 20210099.
Taariikhda oggolaanshaha iyo wax ka beddelka tilmaamaha:
Taariikhda ansixinta: Noofambar 18, 2021
